Intermediate

Part:BBa_S03564

Designed by: Lane Heard   Group: iGEM06_Missouri   (2006-08-16)

hixC-pBAD-hixC-RBS-TetF-TT-RE

Construction intermediate. Cloned in pSB1A3 - readthrough transcription detected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 165
    Illegal NheI site found at 1385
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 311
    Illegal BamHI site found at 1531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 705
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 1557
    Illegal NgoMIV site found at 1925
    Illegal NgoMIV site found at 2085
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None